Biological Treatment With Consortia Of Mn And Fe –Oxidizing Bacteria For Water Purification: Characterization And Prospect Of Process And Product

Keywords: biological treatment, Fe-oxidizing bacteria, Mn-oxidizing bacteria, water
purification

Cahyani, Vita Ratri; Ariyanto, Dwi Priyo
LPPM UNS, Penelitian, DP2M, Hibah Kompetitif Penelitian Strategis Nasional, 2009

Groundwater is one of the main water resources for society. Most of the available wells indicated the increase of Mn(II) and Fe(II) concentration more than the allowed value for drinking water. The present study was the first study in exploring diversity of microbiota in Indonesia which was potential to be applied as effective biological oxidant for separating Mn and Fe in water purification. The present study conducted: (1) isolation of Mn and Fe -oxidizing bacteria from Mn and Fe illuvial horizons in paddy fields from 4 types of soils (Oxisol Tuntang, Inceptisol Jogonalan, Vertisol Masaran, Alfisol Jumantono), (2) investigation on the oxidation types of the isolated Mn-oxidizing bacteria, (3) investigation on the oxidation capacity/capability of the isolated Mn and Fe -oxidizing bacteria in the simple design of water purification process. Consortia of Mn and Fe -oxidizing bacteria from the respective of soil type and also from the combination of 4 types of soils indicated the capability in the Mn and Fe deposition/oxidation or in decreasing of soluble Mn and Fe. The consortium of Mn-oxidizing bacteria with type of oxidation of III indicated higher capability in Mn oxidation than the types I and II, whereas the consortium of Fe-oxidation bacteria of Leptothrix strains indicated higher capability in Fe oxidation than Sphaerotilus strains. Combination of consortium of Mn-oxidizing bacteria and consortium of Fe-oxidizing bacteria indicated positive interaction in reducing soluble Mn and Fe. The decrease in soluble Mn in all of the treatments of application of consortia of Mn and Fe -oxidizing bacteria had not reached to the allowed level for drinking water of 0.1 mg/L. The decrease in soluble Fe in all of the treatments of application of consortia of Mn and Fe -oxidizing bacteria had reached to the allowed level for drinking water of 0.3 mg/L, with the exceptions for the applications of consortium Mn-oxidizing bacteria with the type of oxidation of I and the consortium of Fe-oxidizing bacteria of Sphaerotilus strains. Further investigations are needed to determine the form of isolates carrier which is effective to be applied and to determine more complicated design of water purification with controlling of environmental factors, such as aeration and pH to improve the effectiveness of oxidation process and to conduct molecular investigation to identify the selected bacteria