Kloning dan Karakterisasi keseluruhan genom virus hepatitis C genotipe predominan di Jawa Tengah Indonesia

Keywords: HIV, HCV, MSM, narcotics drugs users, HCV NS5B, HCV E1-E2

Prasetyo, Afiono Agung*)
LPPM UNS, Penelitian, DP2M, Hibah Kompetitif Penelitian Sesuai Prioritas Nasional, 2009

Men who have sex with men (MSM) and narcotics drugs users are associated with high risk of Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), and or Hepatitis B Virus (HBV) infection. In ongoing molecular epidemiology study of HIV and HCV in Indonesia in 2009, from 518 serum collected from MSM and narcotics drugs users incarcerated in correctional facilites in Central of Java, the anti-HIV positive serum, anti-HCV positive serum, and HBsAg positive serum were 6.6% (34/518), 24.9% (129/518), and 5% (26/518), respectively. From the anti-HIV positive serum, 13 serum were positive for the HIV RNA. From the anti-HCV positive serum, 56 were positive for the HCV RNA. The HCV NS5B and E1-E2 were sequenced and phylogenetic analyzed. The most dominant HCV subtype of 56 HCV strains studied was 1a, and followed by 3a, 4a, 1b, 1c and 3k. The most prevalence genotipe with higher virus titer in the serum is going to be cloned its all genome. The most prevalence genotipe with higher virus titer in the serum is going to be cloned its all genome.