Key words : Antioxidative activity, DPPH method, Folin-Ciocalteu method, flavonoid.
Elfi Susanti VH, Yandi Syukri
LPPM UNS, DP2M, Penelitian, Hibah Bersaing, 2009
Isolation of bioactive compound from dragon fruit have been investigated. The dragon fruit was extracted with methanol and the extract was concentrated using rotary vacuum evaporator. The extract exhibited strong antioxidant activity in DPPH method with IC50 of 192.667 μg/ml, and total phenol in Folin-Ciocalteu method with 246 ppm/1 Kg dry extract. Organoleptic characterization of methanol extract exhibited pasta form, brown, bitter, and aromatics smell. Thin Layer Chromatography (TLC) with silicagel F254 as stationary phase and mix of n-butanol:acetic acid:water (4:1:5) as mobil phase show that there are 4 spot and the first spot contains flavonoid. The compounds were separated with coloumn chromatography. Characterization of the compound will be done in the second year.