Keywords: biosurfactant, rhamnolipids, cassava flour wastewater, Pseudomonas fluorescens, emulsification activity, emulsification stability
Venty Suryanti, Desi Suci Handayani, Soerya Dewi Marliyana and Desi Ratnaningrum*)
LPPM UNS, Penelitian, DP2M, Publikasi Internasional , 2009
The optimization of the biosurfactant production by Pseudomonas fluorescens using cassava flour wastewater (manipueira) as substrate was evaluated. The best condition of biosurfactant production was obtained when using media containing natural manipuiera, nutrient broth and NaCl for 2 days of fermentation. The biosurfactant was identified as rhamnolipids and could reduce the surface tension of water from 80 mN/m to 59 mN/m at the CMC value of 715mg/L. The emulsion type of purified biosurfactant was water in oil (w/o) emulsion. The interfacial surface tension and E24 of the purified biosurfactant using different hydrocarbons were tested. The emulsification activity and stability of the purified biosurfactants were compared to the Tween 80 and Triton X-100 with soybean oil, lubricant oil and kerosene as water-immiscible substrates. The effect of temperature, pH and salinity on the activity of purified of biosurfactant was also investigated. The ability of P. fluorescens FNCC 007 to produce biosurfactant with efficient emulsification properties could suggest potential use of this product in industrial and environmental applications.