Key words: Nephotettix virescens, active transmitters, DNA banding pattern
by: Supriyadi and Retno Wijayanti
Leafhopper, Nephotettix virescens (Hemiptera: Cicadellidae) is an important role as a vector of rice tungro virus. As a vector, the characters of N. virescens active transmitters have never been studied in detail. The objective of this research was to study the character of N. virescens active transmitters from endemic and nonendemic of rice tungro diseases. The specimens of N. virescens active transmitters were identified by transmitting ability of tungro virus, using Standard Evaluation System (SES) for Rice Tungro Virus of IRRI. The genetic variation of N. virescens active transmitters were determined by using RAPD –PCR method. Three primers, namely OPB01, OPB10, and OPB08 were used to amplify the DNA N. virescens. The result of the research indicated that all N. virescens showed distinct RAPD – PCR electrophoresis types using the three primers. Based on DNA banding pattern, the group among N. virescens did not correlate with their origin, both from endemic or nonendemic areas. The N. virescens collected from Pacitan was not similar and separately from others. The N. virescens from Purwodadi and Ngawi showed similar or
identical, as well as N. virescens from Klaten to Sleman-Yogyakarta.