Production Of Feed Additif Containing Phytase From Recombinant Bacteria For Increasing Food Quality And Meat Of Broiler Chickens

Key Words: Phytse, Feed Additive, Enzyme Extraction, Digestible Nutrient, Carrier, Freezer Dryer

By: Adi Magna Patriadi Nuhriawangsa, Sajidan , Zaenal Bachruddin and Ali Wibowo

The result was used to producted mash phytase from recombinat bacteria BL21 (DE3) EAS1-AMP and its applied to mixed corn soybean diet for broiler chickens. Crude and pure extraceluller phytase was producted with Sajidan method (2002). Induction enzyme to producted phytase with IPTG/Isopropyl-β-D-thiogalactopyranoside (Promega V395D). The in vitro digestible P was determined with Wu et al. (2004). Calculation of digestible P by different of digestible value of P after and before in vitro on dry matter (Tillman et al., 1989). The P containt was determined with Jackson method (1985). Crude phytase extracelluler was mixed wheat corn and hull of rice. Its was leufolized with sun, open and freezer dryer. Stability phytase was determinaned by relative activity enzyme during five weeks (Whitaker dan Stanbury, 1987). Encapsulation enzyme with chitosan. Chitosan was dissolved with acetat acid. The compound of P of hull of rice with phytase and without phytse was high significantly (P<0,01). Wheat rice with phytase had high digestible P than wheat rice without P. In vitro test was shown phytase to increased digestible P 0.002 g, stabilized from pepsin, and unstabilized from pancreas. Chytosan concentration was optimum on 0.8%. Equivalent of chytosan and phytase was optimum on 1:1. Wheat corn more good than hull of rice for carrier on feed additive. Leufolization by freezer dryer more good than oven and sun dryer on feed additive. Phytase on wheat corn and freezer dryer is very good stability during 5 weeks