by: Sri Mulyani, Okid Parama Astirin, Karl-Heinz van Pee
The purpose of this research is to find conditions in growing cultures of Alteromonas luteoviolaceus and isolating their genomic DNA. This is as a first step in research on the theme of identification of halogenase genes from A luteoviolaceus producing antibiotic pentabromopseudilin by hybridization. This research is explorative with single factor design of this study were analyzed qualitatively. The growth of A. luteoviolaceus was performed by making several modifications in the composition of the medium M1 +.
Isolation of genomic DNA of A. luteoviolaceous using two methods: (1) Frenkel method as the usual method for isolation of genomic DNA from bacteria in general [1], [2]; and (2) by Kirby mix procedure of Hopwood et al. [3]. The results showed that the marine bacterium A. luteoviolaceous can be grown in modified M1 + medium in 27-300C for 2-3 days. Isolation of genomic DNA of A. luteoviolaceous was successfully carried out with Kirby mix procedure [3], if the culture was grown in modified M1+ liquid medium for a maximum of 2 days. The growth can be accelerated by placing a spiral on the basis Erlenmeyer used. If the incubation time is more than 2 days it will be difficult to obtain genomic DNA.